不同日龄补饲开食料对湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2016.12.014

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (12): 2441-2449.doi: 10.11843/j.issn.0366-6964.2016.12.014

不同日龄补饲开食料对湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响

刘婷¹，李发弟^1*，王维民^1，2，汪晓娟¹，李冲^1，2，李飞³，郑琛¹，莫负涛¹，王芳彬¹，喇永富¹，李宝胜⁴

（1.甘肃农业大学动物科学技术学院，兰州 730070； 2.甘肃省肉羊繁育生物技术工程实验室，民勤 733300；3.兰州大学草地农业科技学院，兰州 730020； 4.甘肃金昌中天羊业有限公司，金昌 737100）

收稿日期:2015-12-31 出版日期:2016-12-23 发布日期:2016-12-23
通讯作者: 李发弟，教授，博士生导师，E-mail: lifd@lzu.edu.cn
作者简介:刘婷（1985-），女，陕西临潼人，博士生，主要从事反刍动物营养研究，E-mail: lting822@sina.cn
基金资助:
国家自然科学基金（31260564）；国家现代肉羊产业技术体系（CARS-39）

Effects of Starter Feeding on Rumen Papilla Genes Expression Involved in Cellular Growth and Morphology in Hu Lamb at Different Ages

LIU Ting¹，LI Fa-di^1* ，WANG Wei-min^1，2，WANG Xiao-juan¹，LI Chong^1，2，LI Fei³，ZHENG Chen¹，MO Fu-tao¹，WANG Fang-bin¹，LA Yong-fu¹，LI Bao-sheng⁴

(1.College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070，China；2.Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province，Minqin 733300，China；3.College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，China；4.Jinchang Zhongtian Sheep Industry Co.Ltd，Jinchang 737100，China)

Received:2015-12-31 Online:2016-12-23 Published:2016-12-23

摘要/Abstract

摘要：

本试验旨在探讨不同补饲日龄对湖羊羔羊瘤胃形态发育以及瘤胃表皮生长相关基因表达的影响。本试验采用两因素试验设计，设补饲日龄和羔羊日龄两个因子。选择初生重（(3.41±0.27) kg）接近的78只双羔湖羊，0日龄时随机屠宰6只，按照同质性原则将剩余72只湖羊分为7 d补饲组（(3.36±0.15) kg）和42 d补饲组（(3.44±0.44) kg）。7 d补饲组的羔羊从7日龄补饲开食料1，42 d补饲组的羔羊42日龄补饲开食料1，两组羔羊56日龄断奶，60 日龄时开始逐步换开食料2，过渡期10 d。分别在 14、28、42、56、70和84 日龄从两组中各随机挑选6只羔羊进行屠宰，迅速采集瘤胃腹囊，采用Real-time qPCR方法分析瘤胃表皮生长相关基因表达量的发育性变化，并制备切片以观察瘤胃发育。试验结果表明，7 d补饲组羔羊瘤胃乳头宽度显著高于42 d补饲组羔羊（P＜0.05），但瘤胃肌层厚度显著低于42 d补饲组羔羊（P＜0.05）。7 d 补饲组羔羊瘤胃上皮IGFBP3表达量显著低于42 d补饲组羔羊（P＜0.05），瘤胃上皮IGFBP5表达量显著高于42 d补饲组羔羊（P＜0.05）。7 d补饲组羔羊瘤胃乳头长度和宽度与瘤胃上皮TGFβ1和IGFBP3表达量呈显著正相关（R=0.507，P<0.001；R=0.444，P<0.001；R=0.465，P<0.001；R=0.299，P=0.011），与瘤胃上皮IGFBP6表达量呈显著负相关（R=－0.443，P<0.001；R=－0.447，P<0.001）；42 d补饲组羔羊瘤胃乳头长度与IGFBP5表达量呈显著正相关（R=0.227，P=0.018），瘤胃乳头宽度与瘤胃上皮IGFBP3和IGFBP5表达量呈显著正相关（R=0.338，P=0.005；R=0.293，P=0.002）。湖羊羔羊7日龄补饲开食料能促进瘤胃乳头发育，瘤胃乳头生长相关基因调控其发育过程。

Abstract:

The objective of this study was to investigate the effects of starter age on Hu lamb genes expression involved in cellular growth in rumen papilla and rumen morphology.Two factor design (starter age and age of lambs) was adopted in this study.A total of 78 male Hu lambs with similar born body weight （(3.41±0.27) kg） were selected.Of which，six were slaughtered immediately after birth and the remaining 72 were equally divided into 2 groups，a group fed by starter diet 1 at d 7 and a group fed by starter diet 1 at d 42，and lambs from both groups were weaned at d 56 and transacted to starter diet 2 at d 60.The transition period lasted 10 d.Six lambs from each group were slaughtered at d 14，28，42，56，70 and 84，respectively.The ventral sac of the rumen was collected，rumen papilla height，width and muscular thickness were measured，and total RNA was extracted.Real-time qPCR was used to analyze the expression of genes related to rumen papilla growth.Tissue sections were prepared to observe rumen development.The results revealed that rumen papilla width of 7 d-starter group was higher (P<0.05) than that of 42 d-starter group，however，rumen muscular thickness in 7 d-starter group was lower (P<0.05) than that in 42 d-starter group.The IGFBP3 expression of rumen tissue in 7 d-starter group were lower (P<0.05) than that in 42 d-starter group.In contrast，IGFBP5 expression in 7 d-starter group were higher (P<0.05) than that in 42 d-starter group.In 7 d-starter group，rumen papilla height and width were positively correlated with expression of TGFβ1 and IGFBP3 (R=0.507，P<0.001；R=0.444，P<0.001；R=0.465，P<0.001；R=0.299，P=0.011)，while were negatively correlated with expression of IGFBP6 (R=－0.443，P<0.001；R=－0.477，P<0.001).In 42 d-starter group，rumen papilla height was positively correlated with expression of IGFBP5 (R=0.227，P=0.018)，additionally，rumen papilla width were positively correlated with expression of IGFBP3 and IGFBP5 (R=0.338，P=0.005；R=0.293，P=0.002).In conclusion，this study result indicate that starter feeding for lambs at day 7 can improve rumen morphology，and the expression levels of genes involved in cellular growth in ruminal epithelium can regulate rumen development.

中图分类号:

S826
S816

刘婷，李发弟，王维民，汪晓娟，李冲，李飞，郑琛，莫负涛，王芳彬，喇永富，李宝胜. 不同日龄补饲开食料对湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响[J]. 畜牧兽医学报, 2016, 47(12): 2441-2449.

LIU Ting，LI Fa-di，WANG Wei-min，WANG Xiao-juan，LI Chong，LI Fei，ZHENG Chen，MO Fu-tao，WANG Fang-bin，LA Yong-fu，LI Bao-sheng. Effects of Starter Feeding on Rumen Papilla Genes Expression Involved in Cellular Growth and Morphology in Hu Lamb at Different Ages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2441-2449.

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不同日龄补饲开食料对湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响

Effects of Starter Feeding on Rumen Papilla Genes Expression Involved in Cellular Growth and Morphology in Hu Lamb at Different Ages

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